For this study we chose oleic acid as

For this study, we chose oleic Asiatic acid as the 24h continuous inducer for the establishment of lipid accumulating model, which can effectively simulate the pathological process of NAFLD, and we evaluated the roles of COS I and COS II in eliminating lipid accumulation in hepatocytes by detecting the conventional indexes of NAFLD such as the content of TG and lipid droplets in hepatocytes, simultaneously. The results show that the inhibition of TG accumulation by COS I and COS II in the high-dose groups is the most remarkable, with significant dose-dependent responses. Consequently, COS I and COS II present a potential medicinal value in the prevention and treatment of fatty liver. Nevertheless, the molecular mechanisms of inhibiting lipid accumulation in response to COS I and COS II have not been confirmed and will require further exploration in the future.

Neuroglioma, also called gliocytoma, is one of the common malignant tumors in central nervous system at present. Neuroglioma can make aggressive growth around brain tissue, so complete radical treatment can be realized by single excision. Therefore, radiotherapy and chemotherapy are always combined in clinical. However, the curative effects of different chemotherapeutics are always greatly different for its various varieties and combination with different drugs (Johnson et al., 2014; Rogers, 2013). Therefore, the reasonable combination with chemotherapeutics becomes the key to the postoperative researches on patients with neuroglioma. As the current research hotspots, Twist, the anti-apoptosis protein and E-cadherin, the mark of epithelial–mesenchymal transition have been generally accepted for their role playing in carcinogenesis and transfer (Ding et al., 2012; Min et al., 2013). Currently, there has no report on the influences of chemotherapeutics on such protein expression. However, the curative effect will be more persuasive if the role of chemotherapeutics can be explained in aspect of protein level. Therefore, this work further analyzed the difference in Twist and E-cadherin protein expression before and after chemotherapy when studying the curative effects of teniposide with semustine on patients with neuroglioma. The research achievements are presented as follows:

Materials and methods




This work was supported by the Applied Basic Research of Yunnan Province (Kunming Medical University United Project): 2013FB146.

Lumber disc herniation is an important cause of lower back pains. Relevant research indicates that it is generally induced by the degenerated deformation of a disc due to too much labour or spine abnormality. The number of patients with intervertebral disc herniation is increasing. The lumbar discs in the spine make up a structure with a complex shape, which is the hinge and basis of spine activities. They can transfer labour loads, balance the body, stabilize the spine and absorb vibration (Lee and Teo, 2004). All the functions depend on the intact disc. In pathological cases such as disc herniation caused by too much load on the spine, anatomical morphology and biomechanical properties of the spine will have a series of changes.
Recently, there is a growing tendency for disc herniation with non-operation therapies. Chinese massage, usually named “Tuina” in China, is commonly used in China (Zhang et al., 2015; Yang et al., 2014; Hou et al., 2015). Our treatment group proposed new kind of massage technique named “press-extension”, based on improvement of traditional extension massage technique for many years. However, the mechanism of this massage therapy for lumber disc herniation, e.g., the effectiveness and safety of press-extension Tuina, is not clear. Special biomechanical analysis in both lumbar disc herniation and changes of the disc stress–strain distribution states by this treatment is needed.
At present, there are two methods of biomechanical analysis in the lumbar. One is by experimentation and the other is creating a computational model. In vitro experiments conducted using fresh human specimens have inherent limitations (Mehta and Tewari, 2015). For example, human spinal specimens are difficult to get, often with poor bone quality thus not representing the conditions of a living individual. Moreover, there is always the risk of being infected by a virus. Thus, there is need for further models to be used in the field of spinal research. In the analyses of bone joints such as spines, which have complicated shape, load and boundary conditions, finite element (FE) methods can be a useful tool. Using special software, it allows the modelling of complex structures, and demonstrated detailed biomechanical characteristics of lumbar and provides intrinsic parameters (stress, strain, strain energy, etc.).